Greetings From GeneLab!
New Study Mines Data from GeneLab Resulting in Validation of Methods
Antibodies are protein molecules in our bodies that help us resist infectious disease. The Chapes Lab at Kansas State University, in collaboration with the Pecaut Lab at Loma Linda University, are interested in the genes that are expressed and used during the assembly of antibody molecules, and the impact of spaceflight on that process.
Researchers at both labs have analyzed data from the GeneLab Data System, from mice liver tissue in two separate datasets (GLDS-47, GLDS-48) that were generated from mice flown to and housed on the International Space Station. These data were used to help develop the methods – bioinformatic workflow – which were needed to process the data. The GeneLab data were used to validate the workflow and to make comparisons to sequencing data obtained at Kansas State University. The Chapes and Pecaut labs made preliminary comparisons between the immunoglobulin gene used in the spleen compared to the liver, because these data were publically available in the GeneLab Data System.
The results of this study were published in July in the journal Gravitational and Space Research(5(1): 23; http://gravitationalandspacebiology.org/index.php/journal issue. This work was supported by NASA grants NNX13AN34G and NNX15AB45G.
By Stephen Chapes, Principal Investigator, Kansas State University, Chapes Lab
GeneLab added five new studies to the GeneLab Data System for a total of 138 investigations.
- Mouse muscle LC-MSMS upon weightlessness
- Dynamic nature of epigenetic patterns observed during the Mars 520-d mission simulation
- Validation of methods to assess the immunoglobulin gene repertoire in tissues obtained from mice on the International Space Station
- BRIC-23 GeneLab process verification test: Bacillus subtilis transcriptomic, proteomic, and metabolomic data
- Rodent Research-3-CASIS: Mouse liver transcriptomic, proteomic, and epigenomic data
GeneLab at ASGSR, October 25-28, Seattle, WA
GeneLab and Space Biology representatives from NASA Ames Research Center will give three oral presentations and host an exhibit booth at the 33rd annual meeting of the American Society for Gravitational and Space Research.
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