|Space Shuttle Mission STS-126 Mouse Macrophage Transcription Profiling Study
Researchers profile transcriptional changes during differentiation of mouse primary bone marrow cells in microgravity
Primary cultures from mouse bone marrow were induced to differentiate by the presence of recombinant macrophage colony stimulating (rM-CSF) factor for 14-days during spaceflight. Cells were fixed to preserve RNA during flight and returned to Earth for transcriptional profiling using microarray analysis. Complementary analyses included cell proliferation studies and flow cytometry to detect antigens specific to the macrophage lineage.
This investigation was funded by the NASA Space Biology Program Office grant NNX08BA91G and also supported by the American Heart Association grant 0950036G, NIH grants AI55052, AI052206, AI088070, RR16475 and RR17686, the Jerry C. Johnson Center for Basic Cancer Research and the Kansas Agriculture Experiment Station.