Data Release Announcements

July 10, 2016 —
GeneLab releases six datasets from International Space Station Microbial Observatory study ISS environmental sampling targets areas most used by crew members
petri dish with colonies of microbes
Petri dish containing colonies of microbes grown from a sample collected aboard the International Space Station.
Credits: NASA / JPL

NASA's Microbial Payload Tracking (Microbial Observatory-1) is an ongoing census of the microbial community on the International Space Station (ISS). Sampling of the surfaces and atmosphere of the ISS over time is performed by crew members. To evaluate the potential risk to fouling of clean air supplies or contamination of fluids and food, areas that crew members contact daily are targeted for sampling including the dining area, exercise equipment, lavatory, and cupola (the best view in the house). Identifying which microbes flourish in the spaceflight and microgravity environment is important from a crew health perspective given the published findings that pathogenic bacteria become more virulent in this environment. The analysis in these six new datasets was led by Dr. Kasthuri Venkateswaran at NASA's Jet Propulsion Laboratory. More information on the Microbial Observatory-1 series can be found here.

These investigations were funded by the NASA Space Biology Program Office grants 19-12829-26 and 19-12829-27 under Task order NNN13D111T.

July 10, 2016 —
Study designed to identify the microbial diversity of the International Space Station

The ISS has its own environmental microbiome shaped by microgravity, radiation, and limited human presence. To determine the microbial diversity of the ISS, environmental samples were collected from several ISS surface locations from three flight opportunities. Microbe abundance was determined by 16S ribosomal RNA gene (bacteria and archaea) and ITS (fungi) sequencing. A larger goal of this study was to determine the cultivable, total, and viable microbial diversity from the collected ISS surface samples.

  • Study Title: Microbial Observatory (ISS-MO): Microbial diversity
  • Organism: Environmental Samples
  • Data Type: Metagenomic DNA Sequence Data
  • Study Link: genelab-data.ndc.nasa.gov
July 10, 2016 —
Metagenomics study targets functional genomic diversity of ISS microbiome

To further understand how the microbial diversity of the International Space Station changes over time, researchers performed sequencing studies on environmental samples taken from eight different locations on three consecutive sampling sessions. In the study, the main objective was to identify the pool of genes for each location during each sample time to understand the functional and metabolic diversity of microorganisms in the ISS. Identification of the genes was achieved by random DNA sequencing of the pooled samples and mapping to a protein database.

  • Study Title: Microbial Observatory (ISS-MO): Indoor microbiome study of the International Space Station surfaces
  • Organism: Environmental Samples
  • Data Type: Metagenomic DNA Sequence Data
  • Study Link: genelab-data.ndc.nasa.gov
July 10, 2016 —
First study to show the reservoir of antimicrobial genes on the ISS

Tracking of antimicrobial resistance genes is crucial to understanding the risk to for infection and illness to crew working in the closed environment of the ISS. In this study, DNA extracted from each environmental sample was used to create amplicon libraries based on a customized panel of 500 antimicrobial resistance genes followed by next-generation sequencing.

  • Study Title: Microbial Observatory (ISS-MO): Antimicrobial Resistance Genes
  • Organism: Environmental Samples
  • Data Type: DNA Sequence Data
  • Study Link: genelab-data.ndc.nasa.gov
July 10, 2016 —
Study targets pathogenicity of International Space Station microbes

BSL-2 organisms have moderate potential hazard to humans and the environment. The classification includes various microbes that cause mild disease in humans. In this study researchers isolated and characterized bacterial strains from the ISS that showed multiple drug resistance to antibiotics. Whole genome sequencing was performed for 21 strains and is provided in this investigation. Analysis of these strains could lead to further insight of the influence of microgravity on the pathogenicity and virulence of the microorganisms..

  • Study Title: Microbial Observatory (ISS-MO): Study of BSL-2 bacterial isolates from the International Space Station
  • Organism: Environmental samples
  • Data Type: Whole genome DNA sequence data
  • Study Link: genelab-data.ndc.nasa.gov
July 10, 2016 —
Previously uncharacterized Bacillus species identified from samples taken on the ISS

Crew-associated environmental samples were collected from the Kibo Japanese Experiment Module (JEM), US Segment Harmony Node 2, and Russian Segment Zvezda module of the International Space Station and cultured in the laboratory of Dr. Venkateswaran at the Jet Propulsion Laboratory to isolate individual bacterial species. 16S rRNA gene sequencing identified 11 Bacillus isolates belonging to a subgroup of the Bacillus genus known as the B. anthracis/B. cereus/B. thuringiensis group. Whole genome sequence of each of the 11 isolates is provided here.

The overall analysis places these strains defines a previously uncharacterized Bacillus species, now called Bacillus issensis.

  • Study Title: Microbial Observatory (ISS-MO): Molecular characterization of Bacillus issensis, sp. nov., isolated from various quarters of the International Space Station
  • Organism: Bacillus from Environmental Samples
  • Data Type: Whole genome DNA sequence data
  • Study Link: genelab-data.ndc.nasa.gov
July 10, 2016 —
ISS Microbial Observatory: Aspergillus fumigatus strains isolated from the International Space Station

As part of the ISS Microbial Study, researchers identified two Aspergillus fumigatus strains isolated from the HEPA filter and the surface of the cupola of the ISS. Initial whole genome sequence analysis identified the isolates as A. fumigatus. This fungus can cause opportunistic infection termed aspergillosis in individuals with a compromised immune system. A long journey in space may actually compromise the immune system and make astronauts more susceptible to diseases. Researchers also conducted pathogenicity tests using the zebrafish larval model and determined that ISSF-21 is more virulent than two clinical strains (Af293 and CEA10); virulence for strain IF1SW-F4 is still being tested.

In this study, researchers present the draft genome sequences of the two strains, obtained through whole-genome sequencing.

  • Study Title: Microbial Observatory (ISS-MO): Draft Genome Sequence of two Aspergillus fumigatus Strains Isolated from the International Space Station
  • Organism: Aspergillus fumigatus from Environmental Samples
  • Data Type: Whole genome DNA sequence data
  • Study Link: genelab-data.ndc.nasa.gov
June 21, 2016 —
Genes Required for Survival in Microgravity Studied Using Yeast Investigator uses barcode tag to identify surviving yeast strains
yeast spores with barcode image overlay
The common brewer's yeast (Saccharomyces cerevisiae) was used to study the genes required for survival in the spaceflight environment. Thousands of mutant strains were co-cultured to determine which strains could out-compete the others. The winners were identified by virtue of a DNA "barcode" or genetic tag.

Thousands of yeast strains from the Yeast Deletion Collection were flown on Space Shuttle flight STS-135 to the ISS in an experiment to identify genes required for survival in microgravity. Each individual strain was a mutant of the species Saccharomyces cerevisiae (brewer's yeast) that carried a deletion in a single gene. The deletion was marked by a "barcode" DNA sequence to allow identification of the surviving strains after flight. The strains were pooled together to allow competition for growth over approximately 21 generations during the spaceflight experiment. Strains missing from the mix after flight had deletions in genes that were required for survival.

This investigation was funded by the NASA Space Biology Program Office grant NNX10AP01G.

June 21, 2016 —
Collaborative study of plant growth in space conducted by Japanese, Russian and American scientists and astronauts
  • Study Title: RNA-Seq transcriptome analysis of reactive oxygen species gene network in Mizuna plants grown in long-term space flight
  • Principal Investigator: Prof. Dr. Manabu Sugimoto, Okayama University, Japan
  • Organism: Brassica rapa var. nipposinica
  • Data Type: Transcription profiling using RNAseq
  • Study Link: genelab-data.ndc.nasa.gov
June 21, 2016 —
Variable gravity exposure in Zebrafish during early development
  • Study Title: Exposure of zebrafish larvae to relative microgravity between 5-6 days post-fertilization
  • Principal Investigator: Rasoul Nourizadeh, Ph.D.; Norwegian School of Veterinary Science, Oslo, Norway
  • Organism: Danio rerio
  • Data Type: Transcription profiling using microarray
  • Study Link: genelab-data.ndc.nasa.gov
June 21, 2016 —
European experiment examines effects of long duration spaceflight on mouse skin
  • Study Title: Effect of a 91-day long stay in weightlessness on the International Space Station on mouse skin physiology
  • Principal Investigator: Betty Nusgens, Ph.D.; University of Liege, Belgium
  • Organism: Mus musculus
  • Data Type: Transcription profiling using microarray
  • Study Link: genelab-data.ndc.nasa.gov
June 21, 2016 —
Microarray study examines transcriptional responses of yeast grown in microgravity - focus on cell-cell interactions required for mating, biofilm formation, and flocculation
  • Study Title: Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival
  • Principal Investigator: Katty V.Y. Goosens, Ph.D., Free University of Brussels, Belgium
  • Organism: Saccharomyces cerevisiae
  • Data Type: Transcription profiling using microarray
  • Study Link: genelab-data.ndc.nasa.gov
June 21, 2016 —
Rodent transcriptome study spans the gravity continuum
  • Study Title: Transcription profiling of rat response to changes in developmental stage- 3 types of tissue, 3 gravity conditions, 2 developmental conditions
  • Principal Investigator: Karen Plaut, Ph.D.; Purdue University, West Lafayette, IN
  • Organism: Mus musculus
  • Data Type: Transcription profiling using microarray
  • Study Link: genelab-data.ndc.nasa.gov
March 21, 2016 —
Space Shuttle Mission STS-126 Mouse Macrophage Transcription Profiling Study Researchers profile transcriptional changes during differentiation of mouse primary bone marrow cells in microgravity
image of bioserve fpa
The Bioserve Fluid Processing Apparatus (FPA) was used to culture bone marrow cells on STS-126

Primary cultures from mouse bone marrow were induced to differentiate by the presence of recombinant macrophage colony stimulating (rM-CSF) factor for 14-days during spaceflight. Cells were fixed to preserve RNA during flight and returned to Earth for transcriptional profiling using microarray analysis. Complementary analyses included cell proliferation studies and flow cytometry to detect antigens specific to the macrophage lineage.

This investigation was funded by the NASA Space Biology Program Office grant NNX08BA91G and also supported by the American Heart Association grant 0950036G, NIH grants AI55052, AI052206, AI088070, RR16475 and RR17686, the Jerry C. Johnson Center for Basic Cancer Research and the Kansas Agriculture Experiment Station.

  • Study Title: Evaluation of in vitro macrophage differentiation during space flight
  • Principal Investigator: Stephen Chapes, Ph.D; Kansas State University
  • Organism: Mus musculus
  • Data Type: Transcription Profiling using Microarray
  • Study Link: genelab-data.ndc.nasa.gov
March 21, 2016 —
Germinating Fern Spores Help Identify Genes Involved in Sensing and Responding to Gravity Study looks at RNA at critical time point in gravity sensing response
image of spore
A spore from the fern Ceratopteris richardii

During germination, a calcium current is triggered in the fern spore by a gravity sensing mechanism. The calcium current is part of a signalling cascade that orients the growth of the fern. Prior studies have determined that the critical timepoint for the gravity signal in the germination process occurs ten hours after the light signal that causes the onset of germination. In this study, transcriptional analysis was performed at ten hours post-germination to identify genes potentially important for the gravity response.

This study was supported by NASA Space Biology grants NNX09AH45G, NNX09AB41A, and NNX11AF48A to D. Marshall Porterfield and NSF grant IOS-1027514 to Stanley J. Roux

  • Study Title: RNA-Seq analysis identifies potential modulators of gravity response in Ceratopteris spores: Evidence for modulation by calcium pumps and apyrase activity
  • Principal Investigator: Stanley J. Roux, Ph.D.; University of Texas at Austin
  • Organism: Ceratopteris richardii
  • Data Type: RNA-seq reference transcriptome, Ceratoperis, data released by GeneLab
  • Study Link: genelab-data.ndc.nasa.gov
March 21, 2016 —
Additional Data from BRIC-19 Study on Arabidopsis Transcriptome Data from this study were originally released on November 1, 2015 under GeneLab accession number GLDS-37. An additional dataset is now available for this study.
  • Study Title: Comparison of the spaceflight transcriptome of four commonly used Arabidopsis thaliana ecotypes
  • Principal Investigator: Simon Gilroy, Ph.D., University of Wisconsin, Madison
  • Organism: Arabidopsis thaliana
  • Data Type: Transcription Profiling using RNAseq
  • Study Link: genelab-data.ndc.nasa.gov
March 21, 2016 —
Microarray Analysis of Muscle on the Effect of Countermeasures During Bedrest
  • Study Title: Woman skeletal muscle transcriptome with bed rest and countermeasures
  • Principal Investigator: Angele Chopard, Ph.D. Université de Montpellier, Montpelier, France
  • Organism: Homo sapiens
  • Data Type: Transcription profiling using microarray
  • Study Link: genelab-data.ndc.nasa.gov
March 21, 2016 —
European Space Agency Experiment Profiles Transcription of Human Endothelial Cells in Microgravity
  • Study Title: Expression data from SPHINX (SPaceflight of Huvec: an INtegrated eXperiment)
  • Principal Investigator: Silvia Bradamante, Ph.D., Institute of Molecular Science and Technologies, Milan, Italy
  • Organism: Homo sapiens
  • Data Type: Transcription profiling using microarray
  • Study Link: genelab-data.ndc.nasa.gov
March 21, 2016 —
Microarray Study Compares Gene Expression in Astronaut Blood Samples Before and After Shuttle Missions
  • Study Title: Spaceflight Modulates Gene Expression in Astronauts
  • Principal Investigator: Jennifer Barrila, Ph.D., Arizona State University
  • PI Website Links: biodesign.asu.edu
  • Organism: Homo sapiens
  • Data Type: Transcription profiling using microarray
  • Study Link: genelab-data.ndc.nasa.gov
March 21, 2016 —
STS-135 Study Models Immune Response to Spaceflight Using Human Endothelial Cells
  • Study Title: Immune responses to the in vitro LPS assault engineered in the spaceflight multi-omics study
  • Principal Investigator: Meskerem Jibitu, US Army Center for Environmental Health Research, Fort Detrick, MD
  • Organism: Homo sapiens
  • Data Type: Multi-omic analysis
  • Study Link: genelab-data.ndc.nasa.gov
March 21, 2016 —
Ground Study Examines Changes in RNA in Human PBLs Cultured in Rotating Wall Vessels
  • Study Title: microRNA expression profiles in human peripheral blood lymphocytes cultured in modeled microgravity
  • Study Title: (additional): Gene expression profiling of human peripheral blood lymphocytes cultured in modeled microgravity
  • Principal Investigator: Maddalena Mognato, Ph.D., University of Padova, Italy
  • Organism: Homo sapiens
  • Data Type: Transcription profiling by Microarray
  • Study Link: genelab-data.ndc.nasa.gov
  • Study Link: (additional): genelab-data.ndc.nasa.gov
December 16, 2015 —
First Release of Epigenomic Data by GeneLab Omics analyses of mouse liver in microgravity on the Rodent Research-1 payload
liver section
The Habitat module of the Rodent Research Hardware System, shown with both access doors open.

Credits: NASA / Dominic Hart

Liver is the metabolic hub of the vertebrate organ system and is involved in detoxification, regulation of glycogen storage, protein synthesis, and digestion, among other functions. Previous spaceflight experiments have demonstrated many changes in liver gene expression and in the activity of liver enzymes. GeneLab engaged in a sample sharing mission with the Rodent Research-1 (RR-1) project (GLDS-48) and with NASA's ISS National Lab managed by CASIS (GLDS-47) to provide tissue processing and extensive omics analyses on liver tissue from mice flown in microgravity. The RR-1 mission comprised the maiden voyage and validation of NASA's Rodent Research Hardware System. The RNA, protein, and DNA methylation data sets released here complement previous omics analyses from rodents in spaceflight and can be part of longitudinal studies for future rodent missions.

For the NASA investigation, samples were provided to GeneLab by the Rodent Research-1 project. The investigation was funded by the NASA Space Biology Program Office, Space Life and Physical Sciences Research and Applications Division, and additional funding from the International Space Station Research Integration Office to the Space Biology GeneLab Project.

For the National Lab investigation, samples were provided to GeneLab by Dr. Sam Cadena (Novartis Institutes for Biomedical Research) through the Rodent Research-1 project. This investigation was funded by the Center for Advancement of Science in Space (CASIS), the NASA Space Biology Program Office, Space Life and Physical Sciences Research and Applications Division and additional funding from the International Space Station Research Integration Office to the Space Biology GeneLab Project.

  • Study Title: Transcriptomics, Epigenomics and Proteomics of Mouse Liver from the Rodent Research-1 Spaceflight Payload
  • Principal Investigator: Ruth Globus (RR-1 Project Scientist), CASIS National Lab, GeneLab
  • PI Website Links: lsda.jsc.nasa.gov, nasa.gov
  • Organism: Mus musculus
  • Data Type: RNASeq, Whole Genome Bisulfite Sequencing, Proteomics
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
Space Shuttle Mission STS-135 Rodent Liver Transcriptomics Study Researchers investigate the changes in gene expression patterns in mice flown on STS-135 using transcription profiling methods
liver section
Coherent anti-Stokes Raman scattering spectroscopy of liver from mice flown aboard STS-135 compared with AEM ground controls show significantly more lipid accumulation, consistent with the up regulation of genes involved in triglyceride biosynthesis and lipid droplet formation measured in the flight mice.

This study provides a ground to microgravity comparative gene expression data set of female C57BL/6J mice utilizing transcriptional microarray technology. This data release in conjunction with data from previous studies in spleen and thymus using mice flown on the same mission, will allow researchers to perform network analyses that will help to gain a better understanding of the precise mechanisms that result in changes and possible health consequences associated with spaceflight.

This study was supported by the NASA Cooperative Agreement NNX10AJ31G “Cooperative Research in Proton Space Radiation", the LLUMC (Loma Linda University Medical Center) Department of Radiation Medicine and the University of Colorado Anschutz Medical Center Department of Anesthesiology. Liver samples were obtained through the NASA Biospecimen Sharing Program.

  • Study Title: STS-135 Liver Transcriptomics
  • Principal Investigator: Dr. Michael Pecaut
  • Organism: Rodent
  • Data Type: Transcription Profiling Data
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
New Data Available on the GeneLab Data System: Researchers Compare International Space Station Environmental Microbiome with That of Earth-Based Cleanrooms Researchers use whole genome sequencing methods to investigate whether the viable microbiome associated with the International Space Station varies from the viable microbiomes of cleanrooms on Earth
diagram of taxonomic profiles
Hierarchical clustering of samples using taxonomic profiles at the genus level. The taxonomic profiles are clustered based on sampling location. The color scale reflects log-normalized proportional values (e.g. - 1~10%, -2~1%, -3~0.1%). Rows and columns are clustered independently using the furthest neighbor algorithm with a Euclidean distance metric. o: order; f: family; g: genus

Built environments like the ISS are known to have their own microbiomes. Next-generation sequencing methods are being used to explore the ISS microbial profile to enable the development of appropriate safety and maintenance practices. This study provides strong evidence that specific human skin-associated microorganisms constitute a significant population of the ISS microbiome, generating notable differences between the ISS microbiome and cleanrooms on Earth.

This research was funded by NASA Space Biology Grant no. 19-12829-27 under Task Order NNN13D111T award to K. Venkateswaran.

  • Study Title: Microbiomes of the Dust Particles Collected from the International Space Station and Spacecraft Assembly Facilities
  • Principal Investigator: Dr. Kasthuri Venkateswaran
  • Organism: Environmental Samples
  • Data Type: DNA Sequence Data
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
Dr. Sarah Wyatt of Ohio University Partners with GeneLab on Arabidopsis RNA and Protein Profiling Study Researchers investigate differences in gene and protein expression in Arabidopsis seedlings when grown in space and on the ground.
photo of deintegration process
Colin Kruse (Ohio University) and Susan Manning-Roach (Kennedy Space Center), in a cold room at Kennedy Space Center, deintegrating the experiment after the spaceflight.

This study provides a ground-to-microgravity comparative gene expression analysis of Arabidopsis thaliana seedlings. The core project examines global gene expression by RNASeq and the composition of the soluble protein fraction. The GeneLab collaboration augments the core investigation with an additional membrane protein data set. The data will allow researchers to perform network analyses to add to the knowledge of physiological effects of spaceflight during seedling growth.

This competitively selected study was funded by the NASA Space Biology Program Office, Space Life and Physical Sciences Research and Applications Division, NASA Taskbook Grant NNX13AM48G to Sarah Wyatt and additional funding from the International Space Station Research Integration Office to the Space Biology GeneLab Project.

  • Study Title: Proteomics and Transcriptomics Analysis of Arabidopsis Seedlings in Microgravity
  • Principal Investigator: Sarah Wyatt, Ph.D.; Ohio University
  • PI Website Link: wyattlab.wordpress.com
  • Organism: Arabidopsis thaliana
  • Data Type: Mass Spectrometry Assay Data; RNA Sequence Data
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
Dr. Simon Gilroy Partners with GeneLab on the Study of Ecotypic Variation in Plant Transcriptomes Researchers investigate the transcriptional differences of four Arabidopsis ecotypes using RNA sequencing methods
seedlings in petri dish
Seedlings of the Columbia ecotype of Arabidopsis thaliana grown onboard the International Space Station and fixed at 8 days in RNAlater.

The core study characterizes transcriptional patterns of Arabidopsis thaliana induced during germination and growth on the International Space Station. The GeneLab collaboration allows the comparative study of three additional ecotypes. This investigation will aid researchers in assessing the common and ecotype-specific effects of spaceflight on gene expression and will facilitate cross-study data comparisons with future experiments utilizing these strains. This data release includes 48 out of 56 sample expression files with the remaining 8 files to be released at a later date.

This competitively selected study was funded by the NASA Space Biology Program Office, Space Life and Physical Sciences Research and Applications Division, NASA Taskbook Grant No. NNX13AM50G to Simon Gilroy and additional funding from the International Space Station Research Integration Office to the Space Biology GeneLab Project.

  • Study Title: Comparison of the spaceflight transcriptome of four commonly used Arabidopsis thaliana ecotypes
  • Principal Investigator: Dr. Simon Gilroy; University of Wisconsin - Madison
  • PI Website Link: botany.wisc.edu
  • Organism: Arabidopsis thaliana
  • Data Type: RNA Sequence Data
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
New Transcription Profiling Data Available on the GeneLab Data System Identification of mechanosensitive genes in osteoblasts by comparative microarray studies using the rotating wall vessel and the random positioning machine

This study investigates the effects of microgravity during spaceflight on bone loss due in part to decreased bone formation by unknown mechanisms. Because it is difficult to perform experiments in space, researchers used ground-based simulators such as the Rotating Wall Vessel (RWV) and the Random Positioning Machine (RPM) to study the microgravity environment. In this study, researchers exposed 2T3 preosteoblast cells to the RWV for 3 days and found that alkaline phosphatase activity, a marker of differentiation, was inhibited. In addition, they found 61 genes downregulated and 45 genes upregulated by more than twofold compared to static 1 g controls, as shown by microarray analysis. These mechanosensitive genes may provide novel insights into understanding the mechanisms regulating bone formation and potential targets for countermeasures against decreased bone formation during spaceflight and in pathologies associated with lack of bone formation.

  • Study Title: Transcription profiling of mouse osteoblasts under static vs simulated microgravity
  • Organism: Mus musculus
  • Data Type: Transcription Profiling Data
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
Simulation Technique Reveals Delay in the Development of Fruit Flies When Exposed to Microgravity

Using an Earth-based microgravity simulation technique that utilizes a high gradient magnetic field to levitate a biological organism, researchers investigated the biological response to weightlessness in D. melanogaster. From these experiments, researchers observed a delay in the development of the fruit flies from embryo to adult. Microarray analysis indicated significant changes in the expression of immune-, stress-, and temperature-response genes.

  • Study Title: Transcription profiling of Drosophila exposed to a levitation magnet for different lengths of time
  • Organism: Drosophila melanogaster
  • Data Type: Transcription Profiling Data
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
Researchers use Genechip Technology to Investigate Effects of Microgravity on Murine Bone Marrow Stromal Cells

This study investigates the effects of microgravity on Murine Bone Marrow Stromal Cells (BMSC) that were flown to the International Space Station. The researchers use Genechip technology to detect differences in cell proliferation and cell-cycle genes between flight and control samples. This study represents the first report on the behavior of the potentially osteogenic murine BMSC in a 3D culture system.

  • Study Title: Genechip analysis of bone marrow osteoprogenitors exposed to microgravity
  • Principal Investigator: Massimiliano Monticone
  • Organism: Mus musculus
  • Data Type: Transcription Profiling Data
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
Bacillus subtilis Spores Exposed to Real Space Conditions

Spores of B. subtilis 168 were exposed to real space conditions and to simulated Martian conditions for 559 days in low Earth orbit mounted on the EXPOSE-E exposure platform outside the European Columbus module on the International Space Station. Upon return, spores were germinated, total RNA extracted and fluorescently labeled and used to probe a custom Bacillus subtilis microarray to identify genes preferentially activated or repressed relative to ground control spores. Using microarray technology, this study reveals a change in expression of stress-related regulons responding to DNA damage.

  • Study Title: Bacillus subtilis spores, PROTECT experiment, Space-exposed and Mars-exposed vs. Earth-control
  • Principal Investigator: Wayne L. Nicholson
  • Organism: Bacillus subtilis
  • Data Type: Transcription Profiling Data
  • Study Link: genelab-data.ndc.nasa.gov
October 29, 2015 —
MESSAGE 2 space experiment with Rhodospirillum rubrum S1H

Total RNA was extracted from R. rubrum S1H grown after 10 days in space flight or after 10 days in simulated ionizing radiation or simulated microgravity. Each microarray slide contained 3 technical repeats.

Researchers investigated both transcriptomic and proteomic changes in R. rubrum S1H cultures after a 10-day flight on the International Space Station and compared results to corresponding ground controls. Ground simulation of space ionizing radiation and space gravity were performed under identical culture setup and growth conditions encountered during the actual space journey. Whole-genome oligonucleotide microarray was used to test the effects of space flight. This study is unique in combining the results from an actual space experiment with the corresponding space ionizing radiation and modeled microgravity ground simulations, which allows distinguishing the different factors acting in spaceflight conditions.

  • Study Title: MESSAGE 2 space experiment with Rhodospirillum rubrum S1H
  • Organism: Rhodospirillum rubrum
  • Data Type: Transcription Profiling Data
  • Study Link: genelab-data.ndc.nasa.gov
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